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Lustnisfabemo lustnisfabemo issues 15 minute. Additional antibodies used, included mouse anti-collagen IV monoclonal antibody (CIV), clone CIV (Zymed ®; Invitrogen, Carlsbad, California, USA), and its secondary; donkey anti-mouse IgG (H + L) antiserum conjugated with Alexa-Fluor 555 (Invitrogen). The slides were counterstained with either 4’,6-diamidino-2-phenylindole (DAPI) and/or TO-PRO-3 (Pierce, Rockford, Illinois, USA), washed, coverslipped, and dried overnight at 4°C.